The radiobiology of cultured mammalian cells

by Mortimer M. Elkind

Publisher: Gordon and Breach in New York

Written in English
Published: Pages: 615 Downloads: 398
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  • Radiobiology.,
  • Tissue culture.

Edition Notes

Statement[by] Mortimer M. Elkind [and] Gordon F. Whitmore.
ContributionsWhitmore, Gordon F., joint author., American Institute of Biological Sciences.
LC ClassificationsQH652 .E39
The Physical Object
Paginationxvi, 615 p.
Number of Pages615
ID Numbers
Open LibraryOL5995197M
LC Control Number66027013

  All mammalian cells studied to date, normal or malignant, regardless of their species of origin, exhibit x-ray survival curves similar to those in figure. Initial shoulder Survival Curves for Various Mammalian Cells in Culture• The D0 of the x-ray survival curves for most cells cultured in vitro falls in the range of 1 to 2 Gy.•. Radiation type LET (keV/μm) Co photon: kVp photon: MeV proton: 10 MeV proton: 14 MeV neutron: 18 MeV carbon: MeV alpha. Production of cell-based vaccines. To produce viral vaccines, candidate vaccine viruses are grown in mammalian tissue culture of cells with a finite lifespan. These cells are typically Madin-Darby Canine Kidney cells, but others are also used including: Vero, Human diploid cell lines WI and MRC 5, PerC6 and PMK cells. The candidate vaccine virus strain will replicate using the mammalian cells. cell culture research, have been added to this latest edition of the handbook. The handbook is intended as a guide rather than an in-depth text book of cell culture and you are encouraged to consult relevant specialised literature to obtain more detailed information. Design and Equipment for the Cell Culture Laboratory Laboratory DesignFile Size: 2MB.

REFERENCES Standard Textbooks. Alper, T. Cellular Radiobiology. Cambridge University Press, Cambridge, Alter, W. A., and Conklin, J. J. "Radiation Casualties.   Mammalian Cell Survival Curve Shoulder Region Shows accumulation of SUB- LETHAL DAMAGE. The larger the shoulder region, the more dose will initally be needed to kill the same proportion of cells. Beyond the shoulder region The D0 dose, or the inverse of the slop of the curve, indicates the relative radiosensitivity. - A dose of X-rays kills a greater proportion of aerated cells than hypoxic cells i.e. more radiosensitive - Immediately, after RT, cells are hypoxic but given time to recover, cells reoxygenate so that they become sensitive to RT again - proportionally the same each time - Long term restructuring and revascularization of the tumour. Cloning efficiency of a sensitive mammalian cell line culture was used as a measure of toxic activity. Buffalo green monkey cells were exposed to .

There are a series of rigorous steps that you must follow in order to culture mammalian cells and this must be conducted in a hood in a tissue culture lab. You must ensure you’re wearing gloves and a lab coat throughout the entire process. Firstly. Clinical Radiobiology Syllabus Radiation Biology is a diverse subject encompassing a broad spectrum of basic and clinical sciences. It is developing rapidly, particularly in areas of clinical relevance. It is recognised that it is important to develop a broad knowledge in basic Radiobiology before the clinical applications become apparent. @article{osti_, title = {Soft x rays as a tool to investigate radiation-sensitive sites in mammalian cells}, author = {Brenner, D J and Zaider, M}, abstractNote = {It is now clear that the initial geometrical distribution of primary radiation products in irradiated biological matter is fundamental to the observed end point (cell killing, mutation induction, chromosome .   Addeddate Identifier TubianaRadiobiology Identifier-ark ark://t5zz Ocr ABBYY FineReader .

The radiobiology of cultured mammalian cells by Mortimer M. Elkind Download PDF EPUB FB2

The Radiobiology of Cultured Mammalian CellS. Mortimer M. Elkind and Gordon F. Whitmore. Prepared under the direction of the American Institute of Biological Sciences for the U.S.

Atomic Energy Commission. Gordon and Breach, New York, xvi + pp., illus. $; to libraries, $32Author: W. Sinclair. Radiobiology of Cultured Mammalian Cells by M. Elkind (Author) ISBN ISBN Why is ISBN important. ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book.

The digit and digit formats both work. COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle.

The book focuses on the processes, methodologies, and approaches involved in mammalian cytogenetics and radiobiology.

The selection first offers information on the nutrition and metabolism of cultured mammalian cells, blood technique and human chromosomes, and DNA replication in human chromosomes. Author: Michael C.

Joiner,Albert J. van der Kogel; Publisher: CRC Press ISBN: Category: Medical Page: View: DOWNLOAD NOW» Basic Clinical Radiobiology is a concise but comprehensive textbook setting out the essentials of the science and clinical application of radiobiology for those seeking accreditation in radiation oncology, clinical.

Radiosensitivity and the oxygen effect for mammalian cells cultured in vitro in stationary phase. HeLa cells grown in medium supplemented with 15 per cent human serum show increased radiosensitivity when irradiated in unfed confluent culture (“stationary phase”), compared with their response when irradiated during exponential growth (“ by: RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS R.

Rasmussen. Rasmussen From the Exobiology Division, Ames Research Center, National Aeronautics and Space Administration, Moffett Field, California. Painter's present address is the Laboratory of Radiobiology, University of California Medical Center, San Francisco Cited by: Cellular-radiobiology is a well-developed discipline, dating to almost 90 yr ago, when it was first recognized that exposure to ionizing radiation had biological consequences.

Much information has since been accumulated from studies with irradiated animals and plants and, over the last 50 yr in particular, from studies of effects at the cellular, physical, and chemical levels.

time. For mammalian cells growing in culture the S phase is usually in the range of 6–8 h, the M phase less than an hour, G 2 is in the range of 2–4 h and G 1 is 1– 8 h, making the total cell cycle of the order of 10–20 h.

In contrast, the cell cycle for stem cells in certain tissues is File Size: KB. Mammalian cell culture is at the core of biomanufacturing therapeutic proteins and viral vaccines.

Find out how mammalian cells are derived and cultivated, and what opportunities this field holds. Cells isolated from animal tissues can be expanded in culture for use as a research tool, for the production of virus vaccines and various.

The impact of basic science radiobiological research is now being recognized of significant importance in clinical radiation oncology. Observations made in the laboratory using animals as'well as tissue culture have led to a better biologic understanding of techniques for altered fractionation, techniques for measuring tumor cell proliferation, the possi­ bilities and limitations.

appear in a monograph entitled The Radiobiology of Cultured Mammalian Cells, by M. M Elkind and G. Whitmore. ELKIND, ET AL. mouse L (14) cells show that the survival curves of most if not all cells comprising an asynchronous log-phase population have thresholds (see also ref.

15). Therefore. A type of DNA synthesis in mammalian cells that is stimulated by ultraviolet light has been studied by means of radioautography and density gradient by: Mammalian Cytogenetics and Related Problems in Radiobiology: Proceedings of a Symposium Held at São Paulo and Rio De Janeiro, Brazil, October Paperback – January 1, by & 0 more.

Cell culture contaminants can be divided into two main categories, chemical contaminants such as impurities in media, sera, and water, including endotoxins, plasticizers, and detergents, and biological contaminants such as bacteria, molds, yeasts, viruses, mycoplasma, as well as cross- contamination by other cell lines.

• Survival curve for HeLa cells in culture exposed to x-rays • All mammalian cells, normal or malignant, regardless of their species of origin, exhibit similar x-ray survival curves Cell survival curve: multi-target model •Multi-target single hit model: assume the cell has n of surviving cells to 37% of its targets to be „hit‟ for the.

Lockart RZ, Elkind MM, Moses WB: Radiation response of mammalian cells grown in culture. Survival and recovery characteristics of several subcultures of HeLa S 3 cells after x-irradiation. J Natl Cancer InstPubMed Google Scholar. In this article recent progress in the radiobiology of rapidly dividing mammalian cells in culture is discussed.

Special emphasis is placed on the lethal, mutagenic and carcinogenic effects of. The radiobiology of cultured mammalian cells [by] Mortimer M. Elkind [and] Gordon F. Whitmore. In addition, the technological advance that this curve represented convinced me that Sutton and I should convert our laboratory from yeasts to mammalian cells in culture.

To test for the repair of sublethal damage, we performed a series of experiments involving dose fractionation, one of which is shown in Fig. by: 2. Handbook of Radiobiology - CRC Press Book. This handbook presents the most current information on the effects of ionizing radiation on mammalian cells, with emphasis on human tissues.

The dose-effect relationship is emphasized in a quantitative manner. The book contains up-to-date data on the late effects of low levels of radiation on humans.

This note describes the following topics: Cells Under Microscopes, Types of cells, Preliminary Glimpse at Organelles, Preliminary Glimpse at Cytoskeleton, Models for Cell Biology Research, Cell division, Genes.

This book provides a comprehensive overview of some of the advanced cell culture methodologies and applications. We have measured inactivation and mutation induction in cultured mammalian cells exposed to various radiations covering a range of linear energy transfer (LET; ref.

1).Cited by: Although clonogenic cell survival assays were initially described for studying the effects of radiation on cells and have played an essential role in radiobiology, they are now widely used to examine the effects of agents with potential applications in the clinic. These include, in addition to ionizing radiation, Cited by: In mammalian cells, different pathways in response to DNA damage maintain genome stability controlling either the cell cycle or the repair of the damage itself.

These mechanisms are known as the DNA damage response (DDR), which includes several pathways that are selectively activated depending on the genetic insult (reviewed in [ 1 ]).Cited by: 2.

PROGRESS in theoretical radiobiology depends very largely on the interpretation of dose-response curves; nowadays, interest is being focused on the reproductive survival of mammalian by: Written by practicing, active radiobiologists, the book brings together basic laboratory research and practical, clinical applications.

The easy-to-read text and informative illustrations ensure. In print sincethis seventh edition of Radiobiology for the Radiologist is the most extensively revised to date.

It consists of two sections, one for those studying or practicing diagnostic radiolo, nuclear medicine and radiation oncology; the other for those engaged in the study or clinical practice of radiation oncology--a new chapter, on radiologic terrorism, is.

Other members of the group (professor Erik Pettersen) are using mammalian cells in culture. The interests are the control mechanisms working in the cell cycle. Of particular interest is the effect of small radiation doses, given at a low dose rate.

These studies are of importance for environmental problems as well as within cancer treatment. This handbook presents the most current information on the effects of ionizing radiation on mammalian cells, with emphasis on human tissues.

The dose-effect relationship is emphasized in a quantitative manner. The book contains up-to-date data on the late effects of low levels of radiation on : $.

see instruction book Can dialyze against another detergent (e.g., CHAPS). Extraction efficiency is generally > 50% with the cell lines tested (having proteins with up to two transmembrane segments).3 Mitochondria Isolation Kit for Cultured Cells Mitochondria Isolation Kit for Tissue† Mammalian cells Heart, liver, kidney and brain.A parallel approach is the application of the techniques of molecular biology to clone the repair genes in mammalian cells, and to understand genetic defects that alter gene regulation, or to regulate biochemical factors in the cell.

references.}, doi = {/}, journal = {Am. J. Clin. Oncol.; (United States.Cultured mammalian cells were irradiated in single cell suspensions and thin film monolayers at respiration inhibiting (4°C) and at normal cell culturing (37°C) temperatures.

Due to oxygen equilibration and radiolytic depletion problems, the results of the suspension culture experiments were inconclusive.